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. 2021 Jan 7;10:591698. doi: 10.3389/fonc.2020.591698

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Copyright © 2021 Guo, Zou, Zhou, Zhong, He, Huang, Chen, Li, Xiong, Fang and Xiang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NUSAP1 orchestrates the Hippo pathway by stabilizing YAP1 protein. (A) Western blotting analysis to evaluate LATS1/2, YAP1, and YAP1 downstream target genes CTGF and CYR61 in lysates prepared from modified cell lines HGC-27 and BGC823. GAPDH was used as a loading control. (B, C) qRT-PCR analysis of YAP1 and its downstream target genes CYR61, FOXM1, EGFR, and AREG in modified HGC-27 and BGC823 cells. Relative expression is shown over GAPDH mRNA. (D) Western blotting analysis of YAP1 protein in modified BGC823 cells treated with CHX (25 μg/mL) for the indicated times. (E) The line graph shows YAP1 levels normalized to GAPDH at the indicated time points. (F) Immunofluorescence of YAP1 in modified BGC823 cells showing cellular localization. Student’s t test: *p<0.05, **p<0.01.