Skip to main content
. 2021 Jan 7;10:591698. doi: 10.3389/fonc.2020.591698

Figure 6.

Figure 6

YAP1 functions as a downstream mediator of NUSAP1 to accelerate GC cell proliferation and invasion. (A) Western blotting analysis of lysates prepared from NUSAP1-overexpressing GC cells with YAP1 siRNA. GAPDH was used as a loading control. (B) Colony formation assay performed on NUSAP-overexpressing BGC823 and HGC-27 cells with YAP1 knockdown. (C) Graphic representation of the colony numbers under the indicated transfection condition. (D) CCK-8 assay performed on NUSAP-overexpressing BGC823 and HGC-27 cells with YAP1 knockdown. (E) Representative images of fixed and stained Transwell invasion assays performed on modified BGC823 and HGC-27 cells (magnification, ×200). (F) Graphic representation of invaded cell counts from the Transwell assays. (G, H) Cell migration ability evaluated by wound-healing assays performed on modified BGC823 and HGC-27 cells (magnification, ×200). One-way analysis of variance (adjusted for Bonferroni correction test). All data are presented as the mean ± SEM of triplicate experiments, n=3, *P<0.05 and **P<0.01.