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. 2021 Jan 7;11:611170. doi: 10.3389/fpls.2020.611170

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Copyright © 2021 Jasinski, Fabrissin, Masson, Marmagne, Lécureuil, Bill and Chardon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fine mapping of SEN.4 locus. (A) Rosette leaves (rank 4–14) of 7-week-old plants. Upper row: HIF434-Ct; lower row: HIF434-Col. Scale bar corresponds to 2 cm. (B) Percentage of senescent leaves in HIF434-Ct (yellow triangle line) and HIF434-Col (dark green circle line) during the reproductive phase. Flowering transition occurred on average at 23.6 days after sowing (DAS) for HIF434-Col and 24.6 DAS for HIF434-Ct. Stars indicate significant difference between the two genotypes (Student’s test, n = 12, *p < 0.05, ***p < 0.001). (C) The genotype of the most informative recombinants (rHIFs) used to delineate the final 7.875 kb candidate interval is represented with horizontal bars (black for Col-0 allele, white for Ct-1 allele, gray for heterozygous). Dashed vertical bars represent markers used for genotyping between 7.180 and 13.08 Mb on chromosome 4. The phenotype of each rHIF progeny is indicated (phenotype).