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. 2021 Jan 7;11:611170. doi: 10.3389/fpls.2020.611170

FIGURE 2.

FIGURE 2

acd6-2 mutant displays a delayed leaf senescence. (A) Structure of the ACL1 and ACD6 genes with positions of the T-DNA insertions in acl1-1 and acd6-2 mutants is indicated. White boxes represent exons, lines indicate intron, and black boxes represent 5′ and 3′ untranslated regions (UTRs). LB, left border of T-DNA; RB, right border of T-DNA. Gray arrowheads correspond to primers used to genotype mutants. Black arrowheads correspond to primers used for RT-PCR. (B) Reverse transcription PCR (RT-PCR) analysis of ACD6 expression in the fourth leaf of 4-week-old plants of wild type (Col-0), acl1-1, and acd6-2. For each genotype, three different plants (1, 2, and 3) were analyzed. ACTIN was used as a constitutively expressed gene control. Primers used for RT-PCR are indicated by black arrowheads in panel (A). (C) Percentage of senescent leaves in Col-0 (khaki round line), acd6-2 (green triangle line), and acl1-1 (burgundy square line). Stars indicate significant difference between Col-0 and acd6-2 (Student’s test, 7 ≤ n ≤ 8, *p < 0.05, **p < 0.01).