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. 2021 Jan 7;11:600704. doi: 10.3389/fpls.2020.600704

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Copyright © 2021 Deng, Zhang, Yang, Gao, Sheng, Bi, Li, Dong, Yi, He and Hu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Subcellular localization analysis of MaMYB4. The open reading frames of MaMYB4 and ICE1 (used as the nucleus marker) were in framed with GFP and mCherry N-terminus, respectively. The obtained constructs driven by 35S promoter were transient expressed in rice protoplasts and visualized by fluorescence microscopy. The GFP fluorescence signals indicated the localization of nucleus. Overlay images show colocalization of GFP and mCherry signals.