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. 2021 Jan 7;11:600704. doi: 10.3389/fpls.2020.600704

FIGURE 7.

FIGURE 7

MaMYB4 did not physically interact with bHLH, but inhibited the transcriptional activity of CHS, DFR, ANS, and bHLH promoters. (A) The promoter activity assays in the transient expression study revealed that MaMYB4 could repress the activity of CHS, DFR, ANS, and bHLH promoter. We used a dual-LUC reporter plasmid encoding the firefly luciferase (LUC) gene driven by the CHS, DFR, ANS, or bHLH promoter and a Renilla luciferase (REN) gene driven by the constitutive cauliflower mosaic virus (CaMV) 35S. We transiently expressed the pGreen-CHSpro:LUC, pGreen-DFRpro:LUC, pGreen-ANSpro:LUC, and pGreen-bHLHpro:LUC in banana protoplasts together with either 35S:MaMYB4-GFP or 35S:GFP. Different lowercase letters indicate the significant differences among treatments by Student t test at P < 0.001. (B) The yeast two-hybrid assay showing no interaction between MaMYB4 and bHLH. The bait plasmid pGBKT7-ICE1 and the prey plasmid pGADT7-MAPK3 were used as positive control. Meanwhile, the bait plasmid pGBKT7-ICE1 and the prey plasmid pGADT7-MAPK9 were used as negative control.