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. 2020 Nov 25;147(2):517–531. doi: 10.1007/s00432-020-03463-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — PD-1/PD-L1 expression is colocalized and correlated with VISTA expression in PDAC. a Representative colocalization of VISTA and PD-1 proteins in PDAC specimens with immune cells detection were shown. b Representative colocalization of VISTA and PD-L1 proteins in PDAC specimens with tumor cells detection were shown. c, d Positive correlation between the level of VISTA and PD-1 or PD-L1 level in 133 human PDAC specimens. e, f Positive correlation between the expression of VISTA and PD-1 or PD-L1 expression in 177 human PDAC samples from TCGA database. Scale bar = 100 μm (white line at the bottom left)