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. 2021 Jan 7;11:619951. doi: 10.3389/fimmu.2020.619951

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Copyright © 2021 Johnson, Drummer IV, Shan, Shao, Sun, Lu, Saaoud, Xu, Nanayakkara, Fang, Bagi, Jiang, Choi, Wang and Yang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DKO SVF and human macrophage-conditioned medium promote HAEC activation. (A) Stromal vascular fraction (SVF) from 20-week old DKO male mice on NC was cultured overnight in macrophage differentiation medium. Centrifuged supernatant was incubated with human aortic ECs (HAECs) for 24 hours. Untreated HAECs were incubated with EC medium; untreated (n=3), DKO-treated (n=3). (B) Isolated human peripheral blood mononuclear cells (PBMCs) were grown for 7 days in macrophage differentiation medium. Afterwards, cultured HAECs were incubated with centrifuged supernatant (macrophage-conditioned medium) for 24 hours. HAECs in EC medium group were incubated with EC medium only for 24 hours and HAECs in Mac medium group were incubated with macrophage medium only for 24 hours; EC medium (n=11), Mac medium (n=11), Cond Mac medium (n=12). *p<0.05; **p<0.01.