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Kremen2 knockdown induced apoptosis and cell cycle arrest at G2/M phase in GC cells. GC cells transfected with Lenti-shKremen2 or Lenti-shCtrl. (A) Kremen2 knockdown triggered apoptosis of GC cells. Transfected GC cells were stained with Annexin V-APC to detect by flow cytometry. (B) Kremen2 knockdown induced cell arrest of GC cells at G2/M phase. Transfected GC cells were fixed and stained with PI to analyze DNA content by flow cytometry. ***P < 0.001.
