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. 2021 Jan 7;11:613650. doi: 10.3389/fmicb.2020.613650

FIGURE 5.

FIGURE 5

Effect of the OMVs on the biofilm formation by Aeromonas. The degree of the biofilm formation was examined under various coexisting amounts of the OMVs purified from the A. veronii subtype sobria strains, 104 strain (A), 106 strain (D), and 102 strain (E). Addition of Dulbecco’s phosphate-buffered saline (DPBS) alone did not increase the biofilm formation, confirming that DPBS did not affect the biofilm formation by Aeromonas (bar graph labeled DPBS). One-way ANOVA was used followed by Tukey’s test to assess significance. Values are the mean ± standard deviations. *p < 0.05; **p < 0.01. (B) The increase in the amount of the biofilm formed by the addition of the purified OMVs was also confirmed by fluorescently detecting the amounts of eDNAs (by using BOBO-3), proteins (by using SYPRO), and polysaccharides containing GlcNAc (by using WGA-Alexa488). Bars represent 500 μm. The figures showed the results of the experiment using A. veronii subtype sobria 104 strain. (C) The graph shows the results of quantifying the fluorescence intensities of BOBO-3, SYPRO, and WGA-Alexa488 shown in Figure 5B. The fluorescence intensity was quantified using Image J software.