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. 2021 Jan 20;12:472. doi: 10.1038/s41467-020-20792-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Top: Experimental schematic. Middle: Flow cytometry plots representing bi-lineage engraftment in primary transplant (left, week 18 post-transplant) and secondary transplant (right, week 12 post-transplant). Bottom: Bubble plots representing barcode alleles as unique colors from each indicated sorted population. Shown are the three most abundant clones from all six populations. All other barcodes represented as gray bubbles. b Normalized output of barcode alleles with respect to lineage contribution. Total cell output (bar graphs) from indicated barcodes adjusted for both differential lineage output and genome editing efficiency within each subset. Examples of various lineage skewing depicted, with cell counts reflecting relative contributions to the xenograft. One highly engrafted mouse (Mouse 7) depicted of n = 5 total. Skewed output defined as 5-fold or greater bias in absolute cell counts towards lymphoid or myeloid lineages.