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. 2021 Jan 20;11:1850. doi: 10.1038/s41598-021-81413-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The segmentation and analysis for pore measurement in the 3D volumes of cortical bone specimens obtained from SRXTM. (A) 3D volume representatives of the cortical bone at diaphysis of femur from NSD and HSD group are shown in brown color gradient. The segmentation of pore canals inside the bones is rendered in red color enclosed with a transparent background for porosity measurement. All scale bars are depicted 500 µm. (B) quantitative analyses of the porosity of cortical bone at diaphysis of femurs and (C) trabecular bone at proximal metaphysis of tibiae and trabecular bone at distal metaphysis of femurs. The number of animals in each group are indicated in parentheses. *P < 0.05, **P < 0.01 compared to NSD.