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. 2021 Jan 20;11:1850. doi: 10.1038/s41598-021-81413-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Bone histomorphometric analysis of bone resorption markers of tibiae and the mRNA expression levels of osteoblast-derived osteoclastogenic factors in long bone (humerus and radius) of NSD and HSD. (A) Percentages of osteoclast number per bone perimeter (N.Oc/B.Pm) and (B) osteoclast surface per bone surface (Oc.S/BS), and expression of (C) IL-6 and (D) m-CSF. The mRNA expression of each gene was normalized by β-actin expression. Numbers in parentheses are numbers of animals in each group. *P < 0.05, ***P < 0.001 compared to NSD.