Table 1.

Measured K_d values (in nM) of representative natural and artificial K120R-bound and K120R-unbound REs for WT and K120R-p53.

RE name	RE sequence	Kd [nM]
		WT		K120R
		200 mM NaCl	275 mM NaCl	200 mM NaCl	275 mM NaCl
p21	GAACATGTCCcAACATGTTg	26	23	35	43
MDM2	AGACAAGTCaGGACTTaaCT	20	97	36	72
APBB2	AGGCATGTCCcAACATGCCC	10	40	37	80
3Q05 pdb	GGGCATGTCTGGGCATGTCT	6	33	33	78
BAX	GGGCAgGCCCGGGCTTGTCg	22	460	280	N/A
NOXA	GAGCgTGTCCGGGCAgGTCg	45	446	68	∼2900
“Pro-apoptotic”	GGGCATGCCCGGGCATGCCC	15	254	23	∼3500
6FJ5 pdb	AGGCATGCCTAGGCATGCCT	8	783	140	800
BAX_8/13	GGGCAgGTCCGGACTTGTCg	8	40	94	168
NOXA_3/13	GAACgTGTCCGGACAgGTCg	23	60	14	181
p21_8/13	GAACATGCCCcAGCATGTTg	33	99	55	479
BAX_8/13 I/C	GGGCAgG(I-)CCCGGI(-C)CTTGTCg	9	83	150	N/A
6FJ5_8/18 I/C	AGGCATG(I-)CCTAGGCATG(I-)CCT	8	58	72	691

Lower-case letters in RE sequences indicate mismatches from p53 RE consensus. I (inosine) and C (cytosine) in bold indicate I/C bps. Letters in parenthesis indicate a nucleotide of the I-C bp found on the complementary strand. Underlined are core (CWWG) sequences.

N/A not available.