Fig. 2.

Shows the Western blot analysis on HDF, skin fibroblasts (GM00637) and keratinocytes (HACAT) cells.

HDF cell lysates from treated or mock treated cells were immunoblotted with different antibodies as indicated in Fig. 2A, 2C. Actin was used as loading control. The band intensity of the different proteins was quantified using ImageJ Software and the relative increase in expression of various proteins was plotted in Fig. 2B, 2D. The increase in the relative fold intensity was calculated considering untreated cells as 1 fold. Likewise, the result of Western blot analysis on GM00637 has been depicted in Fig. 2E to 2H and that of keratinocytes (HACAT) in Fig. 2I to 2L. Data from one of the three independent experiments with similar results is represented here. CTL- Control.