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. 2021 Jan 7;8:625105. doi: 10.3389/fcell.2020.625105

FIGURE 2.

FIGURE 2

TRIM16 promoted the osteogenic differentiation of hPDLSCs. (A,B) The efficacy of a TRIM16-overexpressing lentiviral vector and a TRIM16 specific shRNA were measured by RT-qPCR. (C) The efficacy of a TRIM16-overexpressing lentiviral vector and a TRIM16 specific shRNA were measured by western blot. (D,E) Alkaline phosphatase activity was performed after osteogenic induction for 7 days (n = 3). (F,G) Alizarin red staining and quantity analysis was performed after osteogenic induction for 14 days. (n = 3) (H) OCN levels were measured by an ELISA assay (n = 3). The mRNA expression of osteo-specific markers (I) RUNX2, (J) Sp7 and (K) COL1A1 was assessed by RT-qPCR (n = 3). (L–O) The protein expression of RUNX2, COL1A1 was assessed by western blot after indicated treatment (n = 3). GM represents general medium, OM represents osteogenic-induced medium. *P < 0.05 vs. the control group, respectively.