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. 2021 Jan 7;8:625105. doi: 10.3389/fcell.2020.625105

FIGURE 4.

FIGURE 4

CHIP was involved in TRIM16-mediated osteogenic differentiation. (A) The schematic flow of LC-MS/MS analysis. (B) Interaction between HSP70 and RUNX2 was examined in hPDLSCs (n = 3). (C,D) Expression of CHIP during osteogenic differentiation of hPDLSCs was examined by western blot (n = 3). (E) The mRNA levels of CHIP regulated by overexpression of TRIM16 (n = 3). (F) Protein levels of CHIP regulated by overexpression of TRIM16 (n = 3). (G) The efficacy of a CHIP-overexpressing lentiviral vector was measured by western blot. (H,I) The expression of RUNX2 and HSP70 regulated by CHIP with or without TRIM16. (J,K) ALP activity and (L,M) Alizarin red staining was performed with indicated transfection after osteogenic induction for 7 days or 14 days (n = 3). *P < 0.05 vs. the control group, respectively.