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. 2021 Jan 7;8:621601. doi: 10.3389/fbioe.2020.621601

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Copyright © 2021 Shu, Zhang, Sun, Pan, He, Cheng, Li and Pei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The MG-63 cells proliferation, osteogenesis, and mitochondrial dynamics. (A) No significant difference in cell proliferation between SLM-UT and SLM-CO surfaces. (B) Upregulated relative gene expression of Runx2 and OCN at 14 days. (C) More continuous mineralization nodules of the SLM-CO surface. (D) Upregulated mitophagy related gene expression of the SLM-CO surface (*P < 0.05). No significant difference existed in mitochondrial biogenesis (PGC-1α, PGC-1β, POLγ, and TFAM), fission (DRP1 and FIS1), and fusion (MFN1 and MFN2) related gene expression (P > 0.05).