Skip to main content
. 2021 Jan 20;12(1):101. doi: 10.1038/s41419-021-03400-9

Fig. 5. PKM2 controls RUNX1 stability.

Fig. 5

A Gene set enrichment analysis (GSEA) of proteins the in PKM2-depleted U937 and NB4 cells measured by MS. B Heatmaps of levels of the indicated transcription factors in NB4 or U937 cells in which endogenous PKM2 was stably knocked out and WT PKM2 (WT) or K270R mutant (KR) were expressed. C, D NB4, U937, and 32DBCR-ABL rescue cell lines were subjected to western blots and the indicated proteins were detected. E The mRNA of RUNX1 was detected by quantitative real-time PCR in NB4, U937, and 32DBCR-ABL rescue cell lines. F Western blot analysis of RUNX1 in U937 cells transfected with shRNA targeting PKM2 and treated with MG132 (2 mM for 4 h) as indicated. G Western blot analysis of whole cell lysates derived from U937 cells stably expressing shRNA empty vector (EV) or shPKM2 and treated with cycloheximide (CHX, 20 mg/mL) for the indicated time points. β-actin was analyzed as an internal loading control. H U937 rescue cell lines were treated with MG132 (2 mM for 4 h) and IP assay were performed using antibody against RUNX1 followed by western blot analysis.