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. 2021 Jan 21;6:27. doi: 10.1038/s41392-020-00388-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Expression of a dominant-negative MAML1 reduced the growth of MEC xenografts. GFP-expressing and dnMAML1-expressing H3118 cells (1 × 10⁶ cells/mouse) were subcutaneously injected to the right flanks of 8–12-week-old immune-deficient NOD.SCID mice (n = 7 each group). Mice were euthanized after a 16-day xenograft study. a–c The tumor volumes were measured daily after tumor cell injection (a), and tumor size (b) and tumor weight (c) were presented at the endpoint. d Representative images of Ki-67 staining of H3118-dnMAML1 and control H3118-GFP xenografts (left bar = 100 μm, right bar = 25 μm) were shown. ImageJ was used to analyze Ki-67-positive cells in IHC staining results. Sections of individual tumors (n = 3) in each group were analyzed. The data were presented as the percentage (%) of Ki-67 positive nuclei to the total nuclei. e The expression levels of GFP, dnMAML1, and HES1 in xenograft tumors were analyzed by western blotting (**p < 0.01; ***p < 0.001)