Fig. 5.
The γ-secretase inhibitor DBZ suppressed the growth of MEC xenografts. a NOD.SCID mice were subcutaneously injected with luciferase-expressing H3118 MEC cells. When the xenografts grew to ~50 mm3, mice were randomly separated and treated with either vehicle only (n = 6) or 5 mg/kg DBZ (n = 6) via intraperitoneal injection daily for 7 days. The tumor volumes were measured daily after treatment (**p < 0.01). b–d Bioluminescent imaging of the xenograft tumors (b), tumor size (c), and tumor weight (d) were presented on the final day of treatment. e Representative images showed Ki-67 immunohistochemical staining of H3118-luc xenografts treated with either vehicle control or DBZ (left bar = 100 μm, right bar = 25 μm). ImageJ was used to analyze Ki-67-positive cells on the sections of individual tumors (n = 3) in each group (**p < 0.01; ***p < 0.001)