Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 20;12:481. doi: 10.1038/s41467-020-20786-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 8 — a–c Splenic naïve CD4⁺ T cells from indicated mice stimulated under Th17 polarizing conditions for 4 days. The frequency of CD4⁺RORγt⁺ Th17 (a) and CD4⁺IL-17A⁺ Th17 (b and c) cells and MFI of RORγt (a) were measured by flow cytometry and summarized. d–f Relative mRNA expressions of RORγt (d) and IL-17A (e) genes in Th17-polarized cells and IL-17A protein in the culture supernatants (f) were measured by RT-qPCR and ELISA, respectively. g Relative mRNA expression of Sox5, RORγt, and IL-17A genes in Th17-polarized cells from indicated mice and the IL-17A protein in culture supernatants before and after Sox5 overexpression. Mean ± SEM are shown, n = 5 independent experiments, unpaired two-tailed Student’s t-test (a, c–g). Experimental mice were between 8 and 12 weeks of age, with no preference to gender and were maintained on a C57BL/6 background. Source data are provided as a Source Data file.