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. 2021 Jan 19;2(1):100185. doi: 10.1016/j.xcrm.2020.100185

Figure 3.

Figure 3

Trained immunity after intravenous BCG vaccination

(A and B) Freshly isolated BAL cells (from the right lung) and monocytes from blood (PBMC.mo) and bone marrow (BM.mo) were stimulated for 24 h with (A) Mtb whole-cell lysate (25 μg/mL) or (B) LPS (0.1 μg/mL) before and 2 weeks after intravenous vaccination with BCG. The data are presented as medium control corrected.

(C) Lactate production as an indicator of metabolic rewiring was measured in 24-h supernatant from LPS-stimulated monocytes purified from blood (PBMC.mo) or BM (BM.mo). See also Figure S1.

(D) Heatmap of H3K27ac reads (red) over BCG-specific peaks. The intensity over the center of the peak ± 12 kb is depicted.

(E) The top GO pathways associated with the nearest genes to dynamic H3K27ac, with adjusted p values.

(F) H3K27ac dynamics at interferon regulatory factor 3 (IRF3) and syndecan 2 (SDC2) locus for PBMC.mo pre- and post-vaccination. The complete list of genes and values can be found at GEO: GSE159046.