Figure 4.

Cytokine production by BAL cells

Freshly isolated BAL cells were stimulated (A) with Mtb whole cell lysate or (B) LPS, before (PRE) and 8 weeks after intradermal (.id) or mucosal (.muc) vaccination (with either BCG, in circles, or MTBVAC, in triangles). Cells were typically collected from the lower right lung lobe (R), the lobe targeted by vaccination, and for mucosally vaccinated animals also from the left lung (L), to establish dissemination of the immune response. The data are presented as medium control corrected (mcc); n = 12 animals/group; Wilcoxon matched-pairs signed rank test. p values are indicated at the top of each graph. See also Figure S2.