Figure 3.

Peripheral immune responses after vaccination

Shown is a characterization of the height and breadth of peripheral immune responses after vaccination.

(A and B) Stacked bar graphs depicting (A) CD4+ and (B) CD8+ T cell cytokine polyfunctionality over time (by group median values) after PPD stimulation.

(C and D) PBMC IFNγ production in response to stimulation with (C) PPD or (D) ESAT6-CFP10 fusion protein, measured by ELISPOT over time.

(E) PPD-specific proliferation of PBMCs, plotted as a stimulation index (the ratio of antigen- over medium control-stimulated values) over time.

(F) T cell numbers in skin biopsies taken (3 days) after intradermal injection of saline (Sal) or old tuberculin (Tub) 8 weeks after vaccination.

(G) PPD-specific cytokine production by T cells from Tub skin biopsies (right panel).

The dotted line in (C) indicates the maximum limit of detection. In (G), + indicates PPD-stimulated samples, and − indicates unstimulated, culture medium-incubated samples as controls. All graphs show 6 animals per group, except for (G), where there are 5 animals for the B.muc, M.id, and M.muc groups. Horizontal lines indicate group medians. Significance of group differences was determined by two-sided Mann-Whitney test adjusted for multiple comparisons. Holms-adjusted p ≤ 0.05 is depicted. Color coding per individual is consistent throughout.