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. 2021 Jan 19;2(1):100187. doi: 10.1016/j.xcrm.2020.100187

Figure 5.

Figure 5

Vaccine-induced humoral immune responses at the pulmonary mucosa

(A) Magnitude of Mtb whole-cell lysate (WCL)-specific immunoglobulin (Ig) type A, G, and M responses in BAL over time.

(B) Mtb WCL-specific Ig levels in the lower right (vaccinated) and lower left (unvaccinated) lung lobe at week 8.

Antibody levels in (A) and (B) are plotted as arbitrary units, as determined by standardization against a reference sample.

(C) Binding of BAL Igs to live Mtb, depicted as the fold change in geometric mean fluorescence intensity (GMFI) of Ig-specific detection antibody signals between week 0 and week 8 post-vaccination.

(D) Correlation between Mtb WCL-specific Ig levels (in arbitrary units) and Ig binding to live Mtb (as fold change compared with control) 8 weeks after vaccination.

For all graphs, n = 6 animals per group. Horizontal lines indicate group medians. Color coding per individual is consistent throughout. Significance of group differences was determined by two-sided Mann-Whitney test adjusted for multiple comparisons. Holms-adjusted p ≤ 0.05 is depicted. Correlations in (D) were calculated with Spearman’s rank order test.