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. 2021 Jan 7;7:615915. doi: 10.3389/fvets.2020.615915

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Copyright © 2021 Zheng, Zhang, Yang, Wu, Wang, Feng, Ombati, Zuo, Yang, Liu, Lai, Luo and Long.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PGE₁ structure and tissue distribution in H. sylvestris. Using activity-guided purification, 1 mg of active component was obtained from 15 kg of H. sylvestris. Structural analysis revealed the active substance to be PGE1. PGE1 tissue distribution in H. sylvestris was analyzed by ELISA. Heads showed the greatest quantity of PGE1 (1 228.36 ng/g body weight) compared with coelom and posterior sucker (PS). Statistically significant differences compared with heads of control group (two-tailed Student t-test) are indicated by ***P < 0.001.