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. 2021 Jan 7;10:600927. doi: 10.3389/fonc.2020.600927

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Copyright © 2021 Liu, Fu, Bian, Fu, Xin, Liang, Li, Zhao, Fang, Li, Zhang, Dionigi and Sun

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Upregulating MAPK8IP1P2 inhibits cancer stem cell characteristics in thyroid cancer cells. (A) Real-time PCR analysis of MAPK8IP1P2 expression in 7 thyroid cancer cells, including 4 PTC cell lines, B-CPAP, BHT101, KTC-1, and K1, and 2 ATC cell lines, CAL-62 and 8305C, and 1 thyroid duct cell carcinoma cells, TT, and a normal thyroid follicular epithelial cell line PTFE. GAPDH was used as endogenous controls. *P < 0.05. (B) MAPK8IP1P2 expression in the vector, MAPK8IP1P2 overexpression scramble, MAPK8IP1P2 shRNA#1, and MAPK8IP1P2 shRNA#2 thyroid cancer cells using real-time PCR. Transcript levels were normalized by GAPDH expression. *P < 0.05. (C) Schematic model of lymphatic metastasis model in vivo. (D) H & E staining analysis of tumors in lymph node from the indicated mice group. (E) The count of tumor cells in the tumor areas of lymph node from the indicated mice group. *P < 0.05.