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. 2021 Jan 7;10:609663. doi: 10.3389/fonc.2020.609663

Figure 2.

Figure 2

PCSK9 promoted migration and invasion and inhibited apoptosis of GC cells in vitro. (A) Relative PCSK9 expression in a panel of GC compared with GES1 cells, as determined by quantitative real-time PCR. (B) Western blotting of PCSK9 expression in eight cell lines; β-actin served as the internal control. (C, D) PCSK9 levels verified by western blotting; β-actin as the internal control. (E) Wound healing assay in SGC-7901 with/without PCSK9 knockdown; scale bar 100 μm. (F) Wound healing assay in MGC-803 cells with/without PCSK9 overexpression; scale bar 100 μm. (G) Transwell migration assay of SGC-7901 with/without PCSK9 knockdown; scale bar 100 μm. (H) Transwell migration assay of MGC-803 with/without PCSK9 overexpression; scale bar 100 μm. (I) Transwell invasion assay of SGC-7901 with/without PCSK9 knockdown; scale bar 100 μm. (J) Transwell invasion assay of MGC-803 with or without PCSK9 overexpression; scale bar 100 μm. (K, L) The cells collected and stained with FITC-Annexin V and propidium iodide; the percentage of apoptotic cells analyzed by flow cytometry assay; representative images presented. Data are expressed as mean ± S.D.; **P < 0.01, ***P < 0.001; WT, wild type; NC, negative control; OE, overexpression.