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. 2021 Jan 7;8:621925. doi: 10.3389/fchem.2020.621925

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Copyright © 2021 Sheng, Tan, Lu, Xiao and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Optimization. (A) The optimization of the concentrations of coating antigen for TRFIA; (B) The optimization of the concentrations of first-antibody for TRFIA; (C) The optimization of the concentrations of Eu³⁺-antibody for TRFIA; (D) The optimization of the concentrations of Na⁺ for TRFIA; (E) The optimization of the pH values for TRFIA; (F) The optimization of the contents of methanol for TRFIA.