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. 2021 Jan 21;12(1):110. doi: 10.1038/s41419-020-03375-z

Fig. 7. Mas knockdown (KD) improved incomplete ubiquitination of VDAC1-P62-ubiquitin complex in HK2 cells treated with PA.

Fig. 7

A Protein expression of VDAC1, P62 and ubiquitin in HK2 cells treated with PA for 24 h. B and C Immunoprecipitation assay showing the interaction among VDAC1, P62, and ubiquitin in HK2 cells with or without PA treatment. D Confocal microscopy images of VDAC1 (red) and ubiquitin (UB) (green) in HK2 cells treated with or without PA, showing more colocalization of VDAC1 and ubiquitin in controls than that in PA-treated cells. E Protein expression of VDAC1, P62 and ubiquitin in WT and Mas KD HK2 cells with or without PA for 24 h. F and G Immunoprecipitation assay showing the interaction among VDAC1, P62 and ubiquitin in WT and Mas KD HK2 cells with or without PA treatment. WT, wild type; Mas KD, Mas knockdown. The experiment was repeated three times.