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. 2021 Jan 7;11:616145. doi: 10.3389/fphys.2020.616145

Figure 6.

Figure 6

Effects of local oxygen enrichment on the gene expression and protein production of critical cytokines in the LV and RV of rats exposed to 4-week hypobaric hypoxia. (A–F) Statistical results for the mRNA expression of HIF-1α, ACE, ACE2, ACE/ACE2, Col1α1, and Col3α1 based on the real-time PCR assays. (G) Quantification of myocardial hydroxyproline concentration based on the alkaline hydrolysis method. (H) Evaluation of myocardial angiotensin II (Ang II) based on ELISA. (I,J) Representative western blotting images for the protein expression of HIF-1α, ACE, ACE2, and GAPDH in the LV and RV. (K–N) Statistical results for the relative protein levels of HIF-1α, ACE, ACE2, and ACE/ACE2 in the LV and RV. All data are expressed as mean ± SD (n = 6). NC, normal control group; HAH, high-altitude hypoxia group; and HAHO, high-altitude hypoxia with oxygen enrichment group. *p < 0.05 vs. the NC group, **p < 0.01 vs. the NC group, ***p < 0.001 vs. the NC group, #p < 0.05 vs. the HAH group, and ###p < 0.001 vs. the HAH group.