Fig. 3.

The influence of Cys328 nitrosylation and glutathionylation on vimentin filament assembly. (A) SDS/PAGE analysis of soluble (S) and insoluble (P) fractions obtained after centrifugation for Vim‐WT, Vim‐SNO and Vim‐SSG before salt addition (–), and after incubation with 0.1 m KCl for 2 h (+/2 h) as well as 16 h (+/16 h). (B) AFM and EM images (C) of Vim‐WT, Vim‐SNO and Vim‐SSG assembled for 2 h in the presence of 0.1 m KCl. Bars: (B) 1 μm; (C) 100 nm. The results are representative of the experiments performed in triplicate.