Figure 4. Post mortem wound tissue analysis of a diabetic BKS.Cg-Dock7^m +/+ Lepr^db/J mouse infected with S. aureus.

A: The immunohistochemical analysis for Mmp9 and DAPI shows a slide of the wound (marked with a star) with the surrounding skin, adipose tissue und panniculus carnosus muscle (arrows).

Scale bar: 1,000 µm. Staining by University Leipzig, image courtesy of MoKi Analytics.

B–E: FISH analysis of the wound tissue allows visualization of the S. aureus distribution. B and C show an example of the outer part of the wound tissue with a biofilm colonizing the surface. In the overview (B), the biofilm is localized on the wound surface (DAPI blue). At higher resolution (C), single FISH-positive bacteria (yellow) are visible, forming a biofilm community. D shows a FISH analysis of deeper layers of the wound tissue with the panniculus carnosus muscle and the subcutaneous fat tissue. In E, bacteria (yellow) are visualized in the wound tissue.

Image courtesy of MoKi Analytics.