Table 2.

Parameters of pressure‐induced inactivation of LDH in muscle homogenates of fish species with different degree of piezophilic adaptation. ^a

Species	P ½, bar	$\mathrm{\Delta }{V}_{\text{PP}}^{{}^{\circ }}$ , mL·mol−1	A 0, % b	ΔG°, kJ·mol−1 c	K eq° c
L. florae	1306 ± 87	−36.3 ± 4.8	7.4 ± 2.2	−4.7	0.148
C. armatus	1336 ± 53	−130 ± 34	0	−17.4	9.0 × 10−4
N. kermadecensis	2143 ± 75	−100 ± 31	7.8 ± 3.6	−21.5	1.7 × 10−4
P. swirei	2413 ± 58	−157 ± 63	6.7 ± 2.4	−37.8	2.4 × 10−7

^aThe values given in the Table were obtained by global fitting of data sets composed of the results of 3–8 individual experiments, as shown in Fig. 1. Each of the combined datasets was scaled to have A ₀ + A _max = 100%. The ‘±’ values show the confidence interval estimated for P = 0.05 from the covariance matrix calculated in the process of nonlinear regression.

^bThe value of A ₀ corresponds to the estimate of the percent of maximal activity retained in the pressure‐promoted conformer. In the case of C. armatus, this value was fixed at 0 during optimization in order to prevent the estimated point of extremum from sliding toward the meaningless negative values of A ₀.

^cThe values of the change in Gibbs energy (ΔG°) and the respective constant of equilibrium (K _eq°) of the apparent transition from the (more active) ‘low‐pressure conformer’ to the pressure‐promoted (low activity) state of the enzyme were calculated from the values of $\mathrm{\Delta }{V}_{\text{PP}}^{{}^{\circ }}$ and P _½ using the relationships in Eqs 2 and (4), (5).