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. 2020 Aug 29;117(12):3835–3848. doi: 10.1002/bit.27536

Figure 1.

Figure 1

Screening the purine and pyrimidine biosynthesis pathways for growth decoupling targets. (a) The sgRNA target genes in the purine and pyrimidine biosynthesis pathways of Escherichia coli. (b) Experimental overview. (c) Ratio of OD and of GFP fluorescence in CRISPRi‐induced and uninduced cultures for each of the screened sgRNA targets after 12 and 24 hr of growth. OD and fluorescence were calculated as the average of three biological replicates. Standard deviations between replicates are shown as error bars. A two‐tailed t test was used to check for significant difference between the growth decoupling strains and the control; *p < .05; **p < .001; ***p < .0001. AICAR, 5′‐phosphoribosyl‐1′‐N‐(5‐amino)imidazole‐4‐N‐carboxamide; AIR, 5′‐phosphoribosyl‐1′‐N‐(5‐amino)imidazole; AMP, adenosine 5′‐monophosphate; ASC, adenylosuccinate; CDP, cytidine 5′‐diphosphate; CMP, cytidine 5′‐monophosphate; CRISPRi, CRISPR interference; CTP, cytidine 5′‐triphosphate; FAICAR, 5′‐phosphoribosyl‐1′‐N‐(5‐formylamino)imidazole‐4‐N‐carboxamide; FGAR, 5‐phosphoribosyl‐1‐N‐formylglycinamide, FGAM, 5‐phosphoribosyl‐1‐N‐formylglycinamidine; GAR, 5‐phosphoribosyl‐1‐N‐glycinamide; GMP, guanosine 5′‐monophosphate; IMP, inosine 5′‐monophosphate; N5‐CAIR; 5′‐phosphoribosyl‐1′‐N‐(5‐amino)imidazole‐5‐N‐carboxylate; OD, optical density; OMP; orotidine 5′‐monophosphate; PRA, 5‐phosphoribosyl‐1‐amine; PRPP, phosphoribosyl diphosphate; SAICAR, 5′‐phosphoribosyl‐1′‐N‐(5‐amino)imidazole‐4‐N‐succinocarboxamide; sdAb, single‐domain antibody; UDP, uridine 5′‐diphosphate; UMP, uridine 5′‐monophosphate; UTP, uridine 5′‐triphosphate; XMP, xanthosine 5′‐monophosphate [Color figure can be viewed at wileyonlinelibrary.com]