Matthews and Newsom (1987) |
Statistical analysis |
Experimental conditions lacked real‐world variable effects |
Blackmore (1989) |
Statistical analysis; large sample size (n > 110) |
Unrealistic wash and drying conditions; used just HPCs; Low concentrations |
Ngeow et al. (1989) |
Realistic seed concentrations |
No statistical comparisons; low limit of detection values; no aerosol sampling. |
Ansari et al. (1991) |
Tested virus and bacteria; statistical analysis; single volunteer for all methods |
Unrealistic handwashing conditions; small sample size |
Hanna et al. (1996) |
Optimized laboratory recovery methods |
No statistical analysis; unrealistic conditions; large seed concentrations |
Patrick et al. (1997) |
Small sample size (n = 7) but large number of replicates (n ≥ 36) |
Towels autoclaved prior to use; no statistical analysis |
Gustafson et al. (2000) |
Advanced statistical analysis; large sample size (n = 99); Randomized study design; high seed concentrations but implemented a realistic handwashing scenario |
No evaluation of real‐world microbial contaminants |
Taylor et al. (2000) |
Statistical analysis used with a 5% confidence level for significance |
Dry times not consistent; volunteers instructed to dry hands until they felt dry |
Montville et al. (2002) |
Model simulations (n = 1000) validated with experimental data (n = 30) |
Data compiled from highly diverse studies with data quality uncertainties |
Harrison et al. (2003) |
Performed statistical analysis; large number of replicates |
Did not compare dryers and towels |
Yamamoto et al. (2005) |
Statistical analysis |
Starting counts sometimes low‐ less than 10; data varied across different hand locations; inconsistent results; complicated indigenous flora parameter‐ no evidence of threat |
Snelling et al. (2011) |
Statistical analysis; used real‐world scenario to contaminate hands (handling raw meat followed by handwashing) |
Data highly variable; comparisons not always significant; failed to test for pathogens |
Gendron et al. (2012) |
Considered background PT contamination impacts |
No statistical comparisons; no risk evaluations |
Margas et al. (2013) |
Statistical analysis; controlled environmental conditions; Large number of volunteers (n = 100) |
Data highly variable among participants |
Best et al. (2014) |
Statistical analysis; multiple tracers used |
High inoculum; paint not representative of real‐world conditions |
Best and Redway (2015) |
Utilized both chemical and microbial tracers |
No statistical analysis; high microbial burden may exaggerate transmission potentials; difficult to control reproducibility of volunteer behaviours; assumed handwashing is suboptimal; Risk not evaluated |
Jensen et al. (2015) |
Statistical analysis; pathogen surrogate tracers |
Lack comparison to WD or JD method; Large data variability; Information lacking on data distributions; Exaggerated bacterial concentrations |
Kimmitt and Redway (2016) |
Statistical analysis; used virus surrogates |
High seed concentrations; Lack comparison of WD vs PT |
Wilcox et al. (2017) |
Statistical analysis; targeted HPC and faecal indicator bacteria |
Small pilot study (26 sampling days, single site, 2 washrooms, up to 5 swabs per locale); Information lacking on data distributions |
Best et al. (2018) |
Statistical analysis; large sample size (n = 120 sampling sessions); Multi‐site, multi‐scenario analysis; targeted faecal indicators and pathogenic bacteria |
Highly variable site conditions; Low concentration differentials; Upper detection limit of 300 CFU; Information lacking on data distributions |
Huesca‐Espitia et al. (2018) |
Statistical analysis; multiple test organisms; VARIOUS air filtration methods |
Low concentrations; data details not shown |
Pitt et al. (2018) |
Statistical analysis; utilized realistic handwashing and drying scenario; identified sampled organisms |
Detailed data not provided; inconsistent drying times; methodology difficult to replicate and may not provide accurate counts |
Mutters and Warnes (2019) |
Statistical analysis; large number of volunteers (n = 80); bacterial tracer and resident flora; controlled and consistent environmental conditions; incorporated handwashing and evaluated drying and no drying variables |
No environment sampling; drying methods may not be realistic |