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. 2020 Apr 16;67(6):1011–1019. doi: 10.1002/bab.1875

FIG. 1.

FIG. 1

EZH2 knockdown in lung cancer cells inhibited chemotaxis of macrophages and decreased CCL5 expression. (A) Transfection efficiency of EZH2‐shRNA1# or 2# in A549 and A129L cells detected by qRT‐PCR. (B) Transfection efficiency of EZH2‐shRNA1# or 2# in A549 and A129L cells detected by Western blot. (C) Effect of EZH2‐shRNA2# on chemotaxis of macrophages was determined by transwell assay (400×). (D) Effect of EZH2‐shRNA2# on mRNA expression of CCL2, CCL5, and CXCL2 were determined by qRT‐PCR. (E) Effect of EZH2‐shRNA2# on protein expression of CCL2, CCL5, and CXCL2 were determined by ELISA. ***P < 0.001.