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. 2021 Jan 5;21(3):179. doi: 10.3892/etm.2021.9610

Figure 3.

Figure 3

Transfection efficiency of the miR-206 mimic and CCND2-plasmid in KGN cells. (A) mRNA expression levels of miR-206 in KGN cells following the transfection with the miR-206 mimic or mimic control were analyzed using RT-qPCR. **P<0.01 vs. mimic control group. (B) mRNA expression levels of CCND2 in KGN cells following the transfection with the control-plasmid or CCDN2-plasmid were analyzed using RT-qPCR. ##P<0.01 vs. control-plasmid group. (C) mRNA expression levels of CCND2 in KGN cells following the different transfections were analyzed using RT-qPCR. **P<0.01 vs. mimic control group; &&P<0.01 vs. miR-206 mimic + control-plasmid group. (D) Protein expression levels of CCND2 in KGN cells following the different transfections were analyzed using western blotting. miR, microRNA; CCND2, cyclin D2; RT-qPCR, reverse transcription-quantitative PCR.