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. 2021 Jan 14;21(3):212. doi: 10.3892/etm.2021.9644

Figure 2.

Figure 2

Knockdown of HMGB1 suppresses the viability of HeLa cells. (A) HMGB1 expression levels were significantly decreased in HMGB1 shRNA-transfected HeLa cells compared with the levels in negative control shRNA-transfected HeLa cells. GAPDH was used as an internal reference gene. (B) The gray value of each band is presented in a histogram. Experiments were performed in duplicate. **P<0.01 vs. shRNA-transfected HeLa cells after 48 h; ##P<0.01 vs. control shRNA-transfected HeLa cells after 72 h. (C) Cell viability was determined using an MTT assay. The results demonstrated that transfection with miR-142-3p inhibitor increased cytoplasmic levels of HMGB1 in HeLa cells. Data are presented as the mean ± standard deviation from three experiments. **P<0.01 vs. negative control shRNA-transfected cells at the respective time-points. HMGB1, high mobility group protein B1; shRNA, short hairpin RNA; OD, optical density; ctrl, control.