Table 2. Steady-State and Transient-State IDET Rates and Heme b Redox Potentialsa.
enzyme | TN@pH 6 [s–1] | IDET [s–1] | E vs SHE [mV] |
---|---|---|---|
CDHAAA | 6.14 ± 0.12 | 50.00 ± 0.10 | 102 ± 4 |
CDHBAA | 0.50 ± 0.03 | 0.40 ± 0.02 | 172 ± 5 |
CDHBBA | 0.04 ± 0.01 | 0.02 ± 0.02 | 169 ± 5 |
CDHABA | 2.05 ± 0.01 | 8.42 ± 0.23 | 110 ± 2 |
CDHBBB | 1.93 ± 0.03 | 4.00 ± 0.01 | 158 ± 2 |
CDHABB | 0.48 ± 0.01 | 0.40 ± 0.01 | 97 ± 4 |
CDHAAB | 0.52 ± 0.01 | 0.40 ± 0.02 | 103 ± 4 |
Comparison of cytochrome c reduction rates (turnover numbers, TN) as an indicator of IDET with transient rates (kobs563) at pH 6.0, along the heme b midpoint redox potentials (E vs SHE).