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. 2020 Dec 24;11(2):517–532. doi: 10.1021/acscatal.0c05294

Table 2. Steady-State and Transient-State IDET Rates and Heme b Redox Potentialsa.

enzyme TN@pH 6 [s–1] IDET [s–1] E vs SHE [mV]
CDHAAA 6.14 ± 0.12 50.00 ± 0.10 102 ± 4
CDHBAA 0.50 ± 0.03 0.40 ± 0.02 172 ± 5
CDHBBA 0.04 ± 0.01 0.02 ± 0.02 169 ± 5
CDHABA 2.05 ± 0.01 8.42 ± 0.23 110 ± 2
CDHBBB 1.93 ± 0.03 4.00 ± 0.01 158 ± 2
CDHABB 0.48 ± 0.01 0.40 ± 0.01 97 ± 4
CDHAAB 0.52 ± 0.01 0.40 ± 0.02 103 ± 4
a

Comparison of cytochrome c reduction rates (turnover numbers, TN) as an indicator of IDET with transient rates (kobs563) at pH 6.0, along the heme b midpoint redox potentials (E vs SHE).