Table 1. .
The primers used in real time RT-PCR analysis
| Genes | Primers name | Sequences | Annealing temperature, °C |
Product length, bp |
|---|---|---|---|---|
| Seh |
Seh-F Seh-R |
5-AGTGCGAGAAGATCAGCGAGAAC-3 5-ATCTTCCGAATCTTAATGTCCAGC-3 |
65 | 193 |
| GAS5 |
GAS5-F GAS5-R |
GAS5-F: 5'-ATTGGCACACAGGCATTAGACAG-3' GAS5-R: 5'-CTTCTTTAAAACTTGCTCCACACAG-3' |
64 | 206 |
| MALAT1 |
MALAT1-F MALAT1-R |
MALAT1-F: 5'-TTCGGAGACAAAGCCATTCGC-3' MALAT1-R: 5'-TTTCTACCGTTTTTCAGCTTCCAG-3' | 65 | 121 |
| PVT1 |
PVT1-F PVT1-R |
PVT1-F: 5'-CCTTGGTGTTCCCCTTTTACTGC-3' PVT1-R: 5'-AACATGGTGAAACCCCGTCTCTAC-3' |
65 | 225 |
| NEAT1 |
NEAT1-F NEAT1-R |
NEAT1-F: 5'-CT GGTCTTGTGGAACTGAACTTAGC-3' NEAT1-R: 5'-AACTCCACATCACTCCTCAGACCAC-3' | 65 | 184 |
| PCA3 |
PCA3-F PCA3-R |
PCA3-F: 5'-CCTT CGT GTTGCTGCCTAAT AT G-3' PCA3-R: 5'-TTTGAGGTCTTGAGAATGGGCAC-3' |
65 | 228 |
| Integrin |
Integrin-F Integrin-R |
Integrin-F: 5'-TGTTTACCACTGATGCCAAGACTC-3' Integrin-R: 5'-CAAATTGATGTTTTTCTGGGATAGC-3' |
64 | 179 |
| B-catenin |
B-catenin-F B-catenin-R |
B-catenin-F: 5'-AT CCCACTAATGTCCAGCGTTT G-3' B-catenin-R: 5'-GTCCTCGTCATTTAGCAGTTTTGTC-3' |
65 | 140 |
| GAPDH |
GAPDH-F GAPDH-R |
GAPDH-F: 5'-GCCAAAAGGGTCATCATCTCTCTGC-3' GAPDH-R: 5'-GGTCACGAGTCCTTCCACGATAC-3' | 64 | 183 |
| Bak |
Bak-F Bak-R |
Bak-F: 5'-CGTTTTTTACCGCCATCAGCAG-3' Bak-R: 5'-ATAGCGTCGGTTGATGTCGTCC-3' |
66 | 154 |
| Bax |
Bax-F Bax-R |
Bax-F: 5'-AGGTCTTTTTCCGAGTGGCAGC-3' Bax-R: 5'-GCGTCCCAAAGTAGGAGAGGAG-3' |
65 | 234 |