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. 2021 Jan 21;26:11. doi: 10.1186/s40001-021-00481-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Bioinformatic analysis of GSE56081. a Heatmap of differentially expressed lncRNAs associated with IVD, red = high expression, green = low expression. b Volcano plot of differentially expressed lncRNAs associated with IVD, green dot represents down-regulated lncRNAs and red dot represents up-regulated lncRNAs in IVD tissue; black dot represents normally expressed lncRNAs. c KEGG pathway analysis for differentially expressed lncRNAs. d The abscissa represents gene ratio, and the ordinate represents enriched terms, the node color showed degree of enrichment ranging from low (green) to high (red), while the node size represented the frequency of the proteins in each enriched pathway group. e Subcellular localization plots of lncRNA NEAT1 displayed by lncATLAS. f Representative image of RNA FISH to confirm lncRNA NEAT1 location in nucleus pulposus cells (bars, 40 μm)