Fig. 4.

PCR replicate positivity rates in positive specimens. a Frequency distribution of the number of PCR replicates positive for methylation in patients without neoplasia (n = 81) and with cancer (n = 136). Each assayed specimen generates a total of 9 PCR replicates, 3 for each gene. b In cases (cancer or no neoplasia) where only one replicate was positive, the proportion for each gene responsible for the positive result. FP false positive, TP true positive, TN true negative, FN false negative