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. 2021 Jan 21;22:69. doi: 10.1186/s12864-021-07381-z

Fig. 2.

Fig. 2

Higher Noise in Samples with More Freeze-Thaw Cycles. From left to right, noise—the randomness in read counts between technical replicates—is estimated using Euclidean distance, RMSE, Pearson correlation, and Spearman correlation. a Box plots of noise for samples that underwent either one or two freeze-thaws. b A linear regression was used to determine the expected noise without freeze-thaw (N0, pink) and the expected change in noise with each additional freeze-thaw (ΔN, green). All estimates are significant (p ≤ 0.05)