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. 2021 Jan 21;22:70. doi: 10.1186/s12864-021-07382-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Phylogenetic relationship among membrane-associated NAC TFs from pearl millet, Setaria italica, Brachypodium distachyon and maize. Multiple sequence alignment of all putative membrane-associated NAC TFs in pearl millet (proteins with “PgNAC” in their names), Setaria italica (proteins with “Seita”), Brachypodium distachyon (proteins with “Bradi”) and maize (proteins with “GRMZM”) was conducted using ClustalW. MEGA 7.0 was used to create phylogenetic trees using the neighbor-joining method with 1000 bootstrap replicates and the p distance method