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(A) Measured spectrum in one pixel from an intralipid phantom containing 10nM of IRDye680RD carboxylate. Using pre-measured basis spectral, spectral unmixing recovered the relative signal contributions from, (B) the dye and (C) background autofluorescence. The resulting fit is shown with the data in (A). Images of serial dilution phantoms show the integrated signal (no spectral unmixing applied) in (D), and the associated fitted contributions of (E) the dye and (F) autofluorescence.
