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The linearity of response to fluorophore concentration of the imaging cryo-macrotome for four fluorophores in intralipid phantoms: (A) NaFL showing both integrated and spectrally unmixed results, (B) LI-COR IRDye 680RD showing both integrated and spectrally unmixed results, and (C) NIR channel measurements of ICG and LI-COR IRDye 800CW carboxylate (acquired separately, no spectral unmixing). (D) provides the lowest detectable concentration for each channel/processing approach.
