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. 2021 Jan 7;7:578892. doi: 10.3389/fmolb.2020.578892

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Copyright © 2021 Lin, Guo, Liu and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection and confirmation of PSR amplicons with the appropriate primer combination. (A) 2% AGE of the PSR amplified products. (B) Observation of real time turbidity for the following primer combinations: HBV-PSR1, HBV-PSR2, and HBV-PSR3. (C) Observation of the PSR-amplified products mixed with SGI under natural light. (D) Analysis of the PSR products by the LFB assay. M: DNA maker DL8000. 1, negative control; 2, Amplification of HBV-PH4. LFB, lateral flow biosensor; SGI, SYBR Green I dye.