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. 2021 Jan 7;7:578892. doi: 10.3389/fmolb.2020.578892

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Copyright © 2021 Lin, Guo, Liu and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Optimal amplification conditions for HBV-PSR assay. The optimal PSR reaction conditions to detect HBV-PH4 were monitored by 2% AGE and real-time turbidity. (A) Incubation temperature optimization. (B–E) Concentration optimization for dNTPs, MgCl₂, betaine and Bst DNA polymerase, respectively. M: DNA maker DL8000; N: negative control.